alkaline comet assay kit Search Results


sta 351 kit  (Cell Biolabs Inc)
86
Cell Biolabs Inc sta 351 kit
Sta 351 Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pm41922308-219-10-12?v=Cell+Biolabs+Inc
Average 86 stars, based on 1 article reviews
sta 351 kit - by Bioz Stars, 2026-07
86/100 stars
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dna damage comet assay kit  (Beyotime)
99
Beyotime dna damage comet assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Dna Damage Comet Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pmc12895629-71-11-16?v=Beyotime
Average 99 stars, based on 1 article reviews
dna damage comet assay kit - by Bioz Stars, 2026-07
99/100 stars
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cometassay single cell gel electrophoresis assay kit  (R&D Systems)
95
R&D Systems cometassay single cell gel electrophoresis assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Cometassay Single Cell Gel Electrophoresis Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pmc09220184-58-13-19?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
cometassay single cell gel electrophoresis assay kit - by Bioz Stars, 2026-07
95/100 stars
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cometassay single cell gel electrophoresis kit  (R&D Systems)
96
R&D Systems cometassay single cell gel electrophoresis kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Cometassay Single Cell Gel Electrophoresis Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pm40030013-414-7-13?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
cometassay single cell gel electrophoresis kit - by Bioz Stars, 2026-07
96/100 stars
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oxiselect comet assay kit  (Cell Biolabs Inc)
86
Cell Biolabs Inc oxiselect comet assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Oxiselect Comet Assay Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pmc12398329-324-19-23?v=Cell+Biolabs+Inc
Average 86 stars, based on 1 article reviews
oxiselect comet assay kit - by Bioz Stars, 2026-07
86/100 stars
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trevigen comet assay kit  (Trevigen)
86
Trevigen trevigen comet assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Trevigen Comet Assay Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pmc12541385-118-6-6?v=Trevigen
Average 86 stars, based on 1 article reviews
trevigen comet assay kit - by Bioz Stars, 2026-07
86/100 stars
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oxiselect single cell gel electrophoresis kit  (Cell Biolabs Inc)
86
Cell Biolabs Inc oxiselect single cell gel electrophoresis kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Oxiselect Single Cell Gel Electrophoresis Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pm41292300-59-1-12?v=Cell+Biolabs+Inc
Average 86 stars, based on 1 article reviews
oxiselect single cell gel electrophoresis kit - by Bioz Stars, 2026-07
86/100 stars
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plus edu alexa fluor 488 flow cytometry kit thermo fisher c10633 comet assay kit trevigen 4250 050 k pierce  (Bio-Rad)
97
Bio-Rad plus edu alexa fluor 488 flow cytometry kit thermo fisher c10633 comet assay kit trevigen 4250 050 k pierce
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Plus Edu Alexa Fluor 488 Flow Cytometry Kit Thermo Fisher C10633 Comet Assay Kit Trevigen 4250 050 K Pierce, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plus edu alexa fluor 488 flow cytometry kit thermo fisher c10633 comet assay kit trevigen 4250 050 k pierce - by Bioz Stars, 2026-07
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cometassay single cell gel electrophoresis assay kit  (Trevigen)
86
Trevigen cometassay single cell gel electrophoresis assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Cometassay Single Cell Gel Electrophoresis Assay Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pmc12781723-68-1-14?v=Trevigen
Average 86 stars, based on 1 article reviews
cometassay single cell gel electrophoresis assay kit - by Bioz Stars, 2026-07
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alkaline comet 288 assay kit  (Trevigen)
86
Trevigen alkaline comet 288 assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Alkaline Comet 288 Assay Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pm41042234-119-3-8?v=Trevigen
Average 86 stars, based on 1 article reviews
alkaline comet 288 assay kit - by Bioz Stars, 2026-07
86/100 stars
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oxiselect comet assay kit  (Fisher Scientific)
86
Fisher Scientific oxiselect comet assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Oxiselect Comet Assay Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
oxiselect comet assay kit - by Bioz Stars, 2026-07
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dna damage comet assay kit  (GBCBIO Technologies Inc)
90
GBCBIO Technologies Inc dna damage comet assay kit
Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced <t>DNA</t> <t>damage</t> response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide
Dna Damage Comet Assay Kit, supplied by GBCBIO Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alkaline+comet+assay+kit/pm40527291-89-9-14?v=GBCBIO+Technologies+Inc
Average 90 stars, based on 1 article reviews
dna damage comet assay kit - by Bioz Stars, 2026-07
90/100 stars
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Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced DNA damage response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide

Journal: Cell & Bioscience

Article Title: The mutual regulation of SOX12 and RNF168 modulates cisplatin resistance in esophageal squamous cell carcinoma cells by regulating DNA damage repair

doi: 10.1186/s13578-025-01505-8

Figure Lengend Snippet: Knockdown of SOX12 in esophageal cancer cells suppresses the cisplatin-induced DNA damage response. A The detection of the efficiency of DNA double-strand breaks repair was performed by co-transfecting HR/NHEJ repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (mCherry) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. *** p < 0.001, **** p < 0.0001. B The detection of the efficiency of DNA NER repair was performed by co-transfecting NER repair detection plasmids and HA-SOX12 or HA-SOX12 (1-280) plasmids into HEK293T cells. After 72 h, flow cytometry was used to detect the expression of green fluorescent protein (GFP) and red fluorescent protein (RFP) in the cells. The statistical analysis is presented in the right panel. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ns, p > 0.05. C Western blot was performed to detect the DNA damage response-related proteins CHK1/p-CHK1, CHK2/p-CHK2, and H2AX/γH2AX in Eca109 cells. These cells were transfected with a control plasmid or shSOX12 plasmids, and were subsequently treated with or without 3µM cisplatin. D Eca109 cells transfected with either control or HA-SOX12 plasmids were treated with 3 µM cisplatin for 12 h, followed by culture in normal medium for 0, 24 and 48 h. Immunofluorescence assay was performed at each time point to assess the expression of γH2AX. The images were captured using a Leica laser confocal microscope. Blue: DAPI; green: γH2AX. E The mRNA expression of SOX12, CHK1/2, H2AX, BRCA1, and PARP1 in Eca109 cells was assessed using the RT-qPCR assay after transfection with control or shSOX12 plasmids. F MG132 rescue assay. Eca109 cells were transfected with control or shSOX12 plasmids and subsequently treated with or without 20 µM MG132 for 6 h. The protein expression of CHK2, H2AX, and SOX12 was subsequently detected using Western blot. G The sensitivity of Eca109 cells and Eca109-CR cells to etoposide were assessed via the CCK-8 assay. H The sensitivity of Eca109-CR cells to etoposide were assessed via the CCK-8 assay following transfection with either the shNC plasmid or shSOX12 plasmid. I Western blotting were performed to detect the DNA damage response-related proteins CHK1/2, p-CHK1/2, H2AX, and γH2AX in Eca109-CR cells. These cells were first transfected with either a control plasmid or shSOX12 plasmids, followed by treatment with or without 20 µM etoposide

Article Snippet: Then cells were harvested and operated according to the instructions of DNA Damage Comet Assay Kit (Beyotime, C2041S).

Techniques: Knockdown, Flow Cytometry, Expressing, Western Blot, Transfection, Control, Plasmid Preparation, Immunofluorescence, Microscopy, Quantitative RT-PCR, Rescue Assay, CCK-8 Assay

Summary figure. Under normal physiological conditions, SOX12 does not exert transcriptional repression on TRIP12 and UBR5. High basal levels of TRIP12 and UBR5 maintain tonic deubiquitination activity, which suppresses RNF168 protein levels and keeps DNA damage repair pathways operating at baseline physiological levels. Upon DNA damage or cisplatin treatment, RNF168 stabilizes SOX12 protein through indirect regulation of its ubiquitination status. Elevated SOX12 protein levels repressed transcriptions of TRIP12 and UBR5. Reduced expression of these deubiquitinating enzymes alleviates the inhibitory effect on RNF168 degradation, leading to a surge in RNF168 protein abundance. This upregulation of RNF168 ultimately enhances cellular capacity for DNA damage repair under genotoxic stress

Journal: Cell & Bioscience

Article Title: The mutual regulation of SOX12 and RNF168 modulates cisplatin resistance in esophageal squamous cell carcinoma cells by regulating DNA damage repair

doi: 10.1186/s13578-025-01505-8

Figure Lengend Snippet: Summary figure. Under normal physiological conditions, SOX12 does not exert transcriptional repression on TRIP12 and UBR5. High basal levels of TRIP12 and UBR5 maintain tonic deubiquitination activity, which suppresses RNF168 protein levels and keeps DNA damage repair pathways operating at baseline physiological levels. Upon DNA damage or cisplatin treatment, RNF168 stabilizes SOX12 protein through indirect regulation of its ubiquitination status. Elevated SOX12 protein levels repressed transcriptions of TRIP12 and UBR5. Reduced expression of these deubiquitinating enzymes alleviates the inhibitory effect on RNF168 degradation, leading to a surge in RNF168 protein abundance. This upregulation of RNF168 ultimately enhances cellular capacity for DNA damage repair under genotoxic stress

Article Snippet: Then cells were harvested and operated according to the instructions of DNA Damage Comet Assay Kit (Beyotime, C2041S).

Techniques: Activity Assay, Ubiquitin Proteomics, Expressing, Quantitative Proteomics